Journal: medRxiv

Article Title: Biallelic WDR91 variants cause a neurodevelopmental disorder through impaired endosomal maturation and autophagy dysregulation

doi: 10.64898/2026.04.03.26349989

Figure Lengend Snippet: A. Time-lapse recording of dynamic changes of the early endosomal marker 2×FYVE-mCherry and the late endosomal protein GFP-Rab7 on endosomes in transduced HEK193 cells (left) and duration of the overlap of 2×FYVE and Rab7 on endosomes (right; one-way ANOVA with Kruskal-Wallis multiple comparison test, ** p<0.01, **** p<10 - ; at least 10 endosomes per cell line were analyzed). B. Colocalization of endogenous EEA1 with Rab7 in WDR91 WT and WDR91 Y15N cells: representative confocal images (left) and assessment of Pearson’s coefficient (right; Mann-Whitney test, *** p<10 - ; at least 30 independent cells per cell line were analyzed). C. EEA1 and Rab7 protein expression in transduced HEK293 cells, representative blot of three independent experiments. Individual fluorescence channels were analyzed separately due to distinct background signal. D. Colocalization of WDR91 with endogenous EEA1 or Rab7 in WDR91 WT and WDR91 Y15N cells: representative confocal images (left) and assessment of Pearson’s coefficient (right; 2-way ANOVA with Sidak multiple comparison test, *** p<10 -3 , **** p<10 -4 ; at least 30 independent cells per cell line were analyzed).

Article Snippet: Cells were then saturated using TBS-Tween 0.01% supplemented with 3% goat serum, and stained with a primary anti-human WDR91 Ab (Abcam) and anti-human EEA1 or anti-human Rab7 Abs (Cell Signaling Technologies) and a secondary goat anti-mouse/rabbit IgG mAb coupled to AF488 or AF687 (Thermo Fisher Scientific).

Techniques: Marker, Comparison, MANN-WHITNEY, Expressing, Fluorescence

Journal: Redox Biology

Article Title: YY1 nitration participates in DbCM cardiomyocyte lipotoxicity by inhibiting ANXA3 -induced microlipophagy

doi: 10.1016/j.redox.2026.104085

Figure Lengend Snippet: ANXA3 participated in T2DM-induced DbCM by regulating microlipophagy. (A-B) Representative images of TEM were used to observe the autophagy and microlipophagy in the hearts of mice. The red arrow represents autophagosomes, and the yellow arrow represents lipid droplets in contact with lysosomes. Bars: 1 μm (C) Relative Rab7a mRNA expression levels in heart tissues of mice analyzed by RT-qPCR, n = 4. (D-E) Relative Rab7a protein expression levels in the heart of mice analyzed by Western blot, n = 4. (F) Relative RAB7A mRNA expression levels in AC16 cells analyzed by RT-qPCR, n = 3-6. (G-H) Relative Rab7a protein expression levels in AC16 cells analyzed by Western blot, n = 3-5. The data were presented as the mean ± SD. ∗ P < 0.05 versus the db/db + OE-GFP group or the si-NC group; # P < 0.05 versus the OE-NC group.

Article Snippet: Blocked with 5% (w/v) non-fat-dried milkat room temperature for 1 h. Then the membranes were incubated with the anti-ANXA3 antibody (Proteintech, 11804-1-AP; 1:1000 [v/v]), the anti-YY1 antibody (Proteintech, 22156-1-AP; 1:1000 [v/v]) the anti-PLIN2 antibody (Proteintech, 15294-1-AP; 1:1000 [v/v]), the anti-SQSTM1/p62 antibody (Cell Signaling Technology, 23214; 1:1000 [v/v]), the anti-LC3 antibody (Cell Signaling Technology, 12741; 1:1000 [v/v]), the anti-Rab7 antibody (Proteintech, 55469-1-AP; 1:1000 [v/v])or the rabbit anti-α-Tubulin antibody (ABclonal, AC031; 1:1000 [v/v])overnight at 4 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Neoplasia (New York, N.Y.)

Article Title: Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors

doi: 10.1016/j.neo.2026.101295

Figure Lengend Snippet: Internalization of cetuximab-vc-MMAF. A. OVCAR8 cells were treated with 10 nM cetuximab-vc-MMAF (red) at 37°C for the indicated times. For time 0 h, cells were incubated on ice for 30 minutes to visualize membrane staining. Nuclei were stained with Hoechst 33342 in all cases. Scale bar: 10 μm. B. Colocalization of cetuximab-vc-MMAF (red) with endocytic markers (green). Cells were treated as above, and colocalization (yellow) with markers for early endosomes (EEA1), late endosomes (RAB7), and lysosomes (LAMP1) analysed at the indicated times. Scale bar: 5 μm. C. Quantitation of colocalization was performed using ImageJ and expressed as a percentage. Data are presented as the mean ± SD of 10 fields per time point, for each vesicle type.

Article Snippet: The antibodies directed to EEA1, RAB7, LAMP1, β-tubulin, cleaved caspase-3, pRb (Ser780), and pH2AX were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Incubation, Membrane, Staining, Quantitation Assay